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This FASEB Science Research Conference discusses genome engineering and new technologies for the precise manipulation of genes and genomes in cellula and in vivo. In addition to its utility for advancing our understanding of basic biology, genome engineering has numerous real-world applications, ranging from correction of disease-causing mutations in humans to engineering plants that better provide fuel, food and industrial raw materials. The first clinical trials and patient treatments using genome engineering approaches are now a reality. The scope of this meeting is expansive, encompassing multiple approaches for modifying genomes a€“ from transgenesis and gene targeting to the creation of synthetic genomes. The experimental models featured include bacteria, fungi, model organisms (e.g.a€”Drosophila, C. elegans, zebrafish, mice, rats), plants, humans, and animals including livestock. We anticipate that this diversity of approaches and experimental systems will create a stimulating meeting environment that will enable new insights and advance the field.