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In the recent years, collaborations between biologists and physicists have generated key discoveries regarding our understanding of bacterial chromosome structure. This conference will recognize the role of inter-disciplinary work and foster new collaborations and ideas. New approaches and technologies have revolutionized the field of bacterial chromosome biology in the last fifteen years. For example, super-resolution microscopy is now being used to visualize previously unresolved structure, genomic tools have enabled the capture of new types of proximity information on a genomic-scale and polymer physics models allow researchers to tackle questions that go far beyond the traditional biological description of chromosome structure.