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Spetses Summer courses are well known for covering various specialized topics within the general fields of molecular and cell biology and also microbiology. This latter field has witnessed a real renaissance in the last twenty years and in this particular FEBS/EMBO Lecture Course with support of IUBMB, we intend to cover different aspects of ‘the New Microbiology’, including bacterial diversity, microbial communities/microbiotas, symbiosis, biofilms, small RNAs or bacterial pathogens.