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Sensor systems have emerged that exhibit extraordinary sensitivity for detecting physical, chemical, and biological entities at the micro/nanoscale. Particularly exciting is the detection and analysis of molecules on miniature devices that have many possible applications in health, environment, analysis, and security. This conference will bring together researchers in the rapidly advancing field of Single Molecule Sensors and nanoSystems. The conference focuses on the most recent advances in micro and nano-sensing techniques that have either demonstrated single-molecule detection or that claim single-molecule detection capability on sensor chips in the longer term.