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The Gordon Research Seminar on Cell Polarity Signaling is a unique forum for graduate students, post-docs, and other scientists with comparable levels of experience and education to present and exchange new data and cutting edge ideas. Cell polarity, or the asymmetric localization of proteins, lipids, and other macromolecules and organelles to specific cellular regions, is essential for biological functions from single cells to complex multicellular tissues. Cell polarity axes are first established, refined, and maintained as an organism undergoes specific developmental programs, giving rise to specific body plans and tissue organization, with disruption of these programs resulting in disease. The focus of this seminar will be on exploring the progression of events underlying cell polarity establishment during developmental, regenerative, and disease programs with particular emphasis on in vivo studies performed in a variety of model organisms and tissues.