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The program covers all aspects of separation sciences in liquid and supercritical fluid phases as well as hyphenation with advanced detection technologies, in particular mass spectrometry. The program will span from fundamentals and theory of chromatographic separations and detection principles, over methodological and technological advances including separation materials, column technologies and instruments, to applications in various fields and quality assurance aspects. The symposium will feature workshops and tutorials, plenary and keynote lectures from the leading scientists in the field. HPLC 2021 will have a big exhibition and vendor seminars in which attendees can see the latest innovations from the leading vendors in the field.