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Chromatin structure, organization and dynamics underlie essentially every aspect of cell function. Rapid advances in recent years in imaging- and sequencing-based methods and in structural tools, such as Cryo-EM, resulted in breakthroughs in our understanding of the spatial and temporal organization of chromatin and its functions. The aim of this workshop is to bring together experienced scientists, Ph.D. students, and postdoctoral fellows working on the functional organization and dynamics of the nucleus and chromatin in diverse cellular processes. Our goal is to establish an informal and intimate atmosphere (120 participants in total), in which oral and poster presentations by students and postdoctoral fellows, from selected abstracts, will be complemented by lectures delivered by leading researchers in the field. The speakers will present exciting recent work, discuss their perspectives on future developments, and highlight the existing conceptual views and technical problems in the area. The lectures will be organized in 8 sessions and will be complemented by 2 poster sessions.