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This EMBO Workshop will continue in the style of a highly successful conference series and will further consolidate the BacNet series as a major international meeting in Europe that attracts leading researchers and young scientists working in the field of molecular microbiology with a special focus on signalling and regulatory networks in bacteria. The majority of cellular functions, such as stress response, information processing and behaviour control are executed by complex networks. However, our understanding of how these networks operate together within a cells, and how they are adaptable for populations and multi-species communities is still limited. This EMBO Workshop will cover most recent advances in research in signaling and regulatory networks in bacteria, and help to shape this rapidly developing field in Europe and worldwide. Progress in the field requires interdisciplinary approaches, combining the tools of molecular biology, biochemistry, biophysics and computational analyses. Advances in the field hold great promise to drive our understanding of bacterial physiology, pathogenicity, ecology and evolution. It will also contribute to the design of networks used in biotechnology and biomedical applications. The workshop will bring together 160 participants, including 23 invited speakers. BacNet21 will offer young researchers ample opportunity to present their work and discuss it with leading scientists.