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EMBO Practical Course — Membrane protein expression, purification and characterization (mPEPC2)

6th September 2021   -   13th September 2021
Hamburg, Germany
https://meetings.embo.org/event/20-mpepc2
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Abstract

Integral membrane proteins (IMPs) are encoded by 20-30% of all open reading frames and provide key functions in the cell. Due to their hydrophobic nature, they are more difficult to study and structural information is lagging far behind their soluble counterparts. This EMBO Practical Course trains PhD students and early career postdocs with the latest developments in membrane protein production, characterization and structure determination methods. Modern protein expression techniques in various hosts will be presented and students will get acquainted with state-of-the-art approaches for studying structural and dynamic aspects of membrane proteins. An attitude to combine protein purification and characterization will be taught to impress the necessity on the students that proteins have to be conditioned with care to achieve structural information. The presence of a crystallization and cryo-EM facility as well as beamlines for SAXS and macromolecular crystallography at the venue will be employed to perform integrated structural biology experiments. The course has an emphasis on practicals in small groups, and a "Meet the scientist" session at the beginning of the course will ensure a close interaction between students and tutors. The aim is to inspire the students to apply the newly acquired knowledge and to maintain interactions among themselves and the tutors afterwards.

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