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Characteristics of a wide range of digital cameras (ie resolution vs sensitivity); Appropriate filter selection for the microscope (ie narrow band vs wide band and imaging techniques to optimize signal localization); Advantages of microscope motorization for digital imaging and 3D rendering (ie Z-stack acquisition); Optical sectioning (ie Apotome and Deconvolution).These topics will all be discussed in lecture format by specialists in the individual fields from both the research field as well as from industry where appropriate. Daily laboratory classes will be included for part of each day to allow for hands on experience with a variety of imaging systems.