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STRANN 2016 is the fifth conference in the series since 2009. The aim of the conference is to establish a communication platform for scientists and engineers involved is research of nanosized objects. Conference covers different scientific fields includning life and material sciences, physics, chemistry, and microscopy. Interactive information exchange helps achieving a better understanding of complex aspects of nanoobject research as far as existing state-of-the art methods and new approaches to overcome current and future challenges.