Gene engineering provides the ability to manipulate gene expression in a desired cell type. In order to realize the full potential of stem cells, the development of tools to modify targeted genes is paramount. This course will provide an overview of three different engineering platforms including CRISPR, TALENs, and ZFNs.The first part of the course will cover the general principles of each of these technologies including design and assembly along with the platforms available and different costs associated with each of them. The second part of the course will transition into different applications including engineering in mice, disease modeling, generating iPSC reporter lines, and high throughput approaches. We will also consider sequencing and quality control considerations for these technologies. Hands on laboratory exercises will accompany lectures to provide training in design, assembly, transfection, and confirmation assays.
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