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RNA-seq or RNA sequencing is a new technology that utilizes the latest in next-generation sequencing approaches to obtain information about the presence/absence as well as the quantity of transcribed RNA (mRNA, rRNA, tRNA, or miRNA). Soon RNA-seq will be transplanting microarrays as the go-to procedure for analyzing the transcriptome of any genome. In this workshop, we will provide hands-on experience with RNA-seq - from the bench to the post-sequencing data acquisition (Illumina NextSeq) and analysis using the latest bioinformatics approaches. With a team of researchers from the NIH, area academic institutions and Illumina, we will cover examples of methodological approaches and applications of RNA-seq analysis to a variety of basic science and clinical biomedical research problems.