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Nucleic acid chemistry represents an important subdiscipline of organic chemistry, since natural and artificial functionalities as well as probes, markers or other biologically active molecules can be introduced synthetically into DNA and RNA, by preparing either the corresponding artificial DNA building blocks or by so-called postsynthetic methodologies. Although nucleic acid chemistry seems to be already a mature research field, actual questions that are raised by research in biology and chemical biology give enough reasons to synthesize new nucleic acid probes and thereby further develop the chemistry. Moreover, beside its biological functionality, DNA and RNA are considered as an increasingly important architecture and scaffold for two- and three-dimensional objects, networks and materials for nanosciences.