A lot of research focuses on cytosine methylation (5mdC) in CpG dinucleotides of DNA. However, the catalogue of known epigenetic modifications in animal DNA has expanded and includes methylation of non-CpG cytosines, as well as three 5mC-derivatives: 5hmdC, 5fdC, and 5cadC. Even more exciting are recent reports demonstrating that adenine can be methylated and exist as N6-methyladenine (6mdA) in animal DNA. The presence of these nucleotide modifications in eukaryotic DNA raises many questions. The meeting will provide opportunities to discuss the current state and identify challenges and future directions related to studying the biological importance of these enigmatic DNA modifications.