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This advanced course on fluorescence super-resolution microscopy will cover the theory and practical application of current super-resolution microscopy techniques to biological questions. The participants will have sufficient time to use current super-resolution microscopes for Localisation Microscopy (e.g. dSTORM /GSDIM) and STED Microscopy with samples prepared during the course. At the end of the course, the participants will be able to plan an experiment using super-resolution microscopy, acquire high quality data at the microscope and analyse the images with state-of-the-art computational methods. Invited guest researchers together with experts from the EMBL and Leica Microsystems will ensure that all aspects are covered in depth.