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The microtubule cytoskeleton is essential for a wide variety of cellular functions, such as chromosome segregation, directed vesicle and organelle transport, cell motility, and cell polarity. Impaired microtubule function can lead to human diseases including cancer and neurodegenerative disorders. In recent years, interdisciplinary approaches embracing cell biology, genetics, molecular biology, biochemistry, biophysics, structural biology and mathematical modelling have made a tremendous impact on the microtubule field.