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Modern EM allows structure-function analysis by combining multidisciplinary approaches with physiological, genetic and biochemical data. Current advances in EM are wide ranging and focus on improving resolution, efficient 3D imaging, molecular mapping, correlative imaging and quantitation. Together they combine to make this approach amongst the most popular in biology. This EMBO Practical Course will provide the students with competence in a wide range of techniques, and then to learn how to integrate these effectively with other approaches in cell biology. Moreover, this EMBO Practical Course offers the opportunity to acquire a portfolio of cutting edge EM methods, allowing the participants to integrate EM tools and readouts into the workflow of their own cell biological research projects.