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The Gordon Research Seminar on Single Molecule Approaches to Biology is a unique forum for graduate students, post-docs, and other scientists with comparable levels of experience and education to present and exchange new data and cutting edge ideas. The focus of this meeting is to facilitate exchange of ideas between junior researchers in an open and stimulating environment. This conference will feature sessions on diverse single-molecule topics such as live cell imaging, single-particle tracking, fluorescence microscopy, and force spectroscopy.