Abstract

Course Overview: This course will provide participants with hands-on tutorials and the theory behind gene transfer into fish embryo, as well as gene knock-in/-out using CRISPR/Cas. Microinjection into zebrafish and medaka embryos will be covered, as well as subsequent screening to identify positive founder fish. Audience: microThis course is aimed at research technicians and scientists interested in the generation of genetic modified fish models. Participants should have basic experience with molecular biology techniques.