Researchers have now uncovered the 3D structure of the RNA enzyme SAMURI. Their study provides insights into the development of ribozymes and the evolution of catalytically active RNA.

RNA molecules are an integral part of the human body: In cells, they ensure the transfer of genetic information and regulate the activity of genes. Some even act as catalysts, allowing chemical reactions to take place that would otherwise take place very slowly or not at all. Enzymes made of RNA are called "ribozymes."
A group of researchers led by Professor Claudia Höbartner at the University of Würburg (JMU) has now uncovered the three-dimensional structure of a very special ribozyme: SAMURI. This is an RNA molecule generated in the lab that the team first presented in 2023. The researchers from the Institute of Organic Chemistry were able to determine the 3D structure of SAMURI using X-ray crystallography and in collaboration with Professor Hermann Schindelin from the Rudolf Virchow Centre in Würzburg.
Small Changes with a Big Impact
What makes SAMURI so interesting for the researchers is its very special ability: the ribozyme can chemically modify other RNA molecules at a specific site and thus influence their function -- for example, activate them or render them recognisable by proteins. Such modifications have some very important tasks in nature and ensure that RNAs can carry out their function properly. If errors occur in this regulation, i.e. if an RNA has too many or too few chemical changes, this can lead to the failure of certain metabolic processes.
"We can think of RNA molecules as sentences made up of individual words and letters (nucleosides)," explains Höbartner. "The smallest changes at individual points -- such as the replacement of a letter -- can completely change the meaning of a word or the entire sentence. Just as the word "bat" becomes "cat" by changing a letter, thus describing two distinct animals with very different abilities, it is similar at the cellular level: "Here, the RNA receives the new information by nature making small chemical changes to it. In science, these are called modifications. Enzymes carry out a chemical reaction on the RNA, using a helper molecule called S-adenosylmethionine, or SAM for short, which is important for many processes in the cell."
SAMURI also uses SAM to introduce modifications in the RNA. It is exciting to note that some natural RNA molecules discovered in bacteria can also interact with SAM -- but without catalysing the chemical reaction. These RNAs are called riboswitches, and they do not chemically modify other RNAs.
This content is taken from University of Würzburg

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"RNA research: Ribozyme structure revealed", MachPrinciple, August 15, 2025, https://machprinciple.com/post/RNA-research-Ribozyme-structure-revealed